2AZD
X-Ray studies on Maltodextrin Phosphorylase (MalP) Complexes: recognition of substrates and CATALYTIC mechanism of phosphorylase family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.779, 105.892, 220.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.830 - 2.160 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24967 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.887 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.800 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.132 | 0.709 |
| Number of reflections | 88911 | |
| <I/σ(I)> | 12.6 | 2.2 |
| Redundancy | 6.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG4000, lithium chloride, TRIS (HYDROXYMETHIL)AMINOMETHANE, MALTOPENTAOSE, SODIUM ORTHOVANADATE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
