2AZ8
HIV-1 Protease NL4-3 in complex with inhibitor, TL-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-07 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.608, 62.608, 81.928 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.200 - 2.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.820 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.210 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.365 |
| Number of reflections | 9295 | |
| <I/σ(I)> | 15.9 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 297.16 | ammonium sulfate, sodium acetate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 297.16K, pH 5.20 |
