2AZ4
Crystal Structure of a Protein of Unknown Function from Enterococcus faecalis V583
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2005-07-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.996, 74.182, 214.624 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.20028 |
Rwork | 0.198 |
R-free | 0.24423 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.392 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.475 |
Number of reflections | 116264 | |
<I/σ(I)> | 12.5 | 1.56 |
Completeness [%] | 95.9 | 77.7 |
Redundancy | 4.1 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | 4% PEG400, 1.7M ammonium sulfate, 0.1M HEPES, pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K |