2AZ3
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum in complex with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.370, 71.520, 157.990 |
| Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.2032 |
| Rwork | 0.200 |
| R-free | 0.27074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nue |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.934 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.063 | 0.289 |
| Number of reflections | 56525 | |
| <I/σ(I)> | 10.14 | 3.32 |
| Completeness [%] | 80.3 | 64.9 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2M MgCl2, 0.1M HEPES-Na, pH7.5, 30% PEG 400, 10mM Co(NH3)6Cl3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
