2AZ2
Flock House virus B2-dsRNA Complex (P4122)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 69.625, 69.625, 154.633 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.600 - 2.600 |
| R-factor | 0.246 |
| Rwork | 0.245 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 12367 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | MPEG 5K, ammomnium sulfate, sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
