2AXY
Crystal Structure of KH1 domain of human Poly(C)-binding protein-2 with C-rich strand of human telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979594 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.603, 115.176, 45.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.21541 |
| Rwork | 0.214 |
| R-free | 0.23759 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.670 | 1.810 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.092 | 0.887 |
| Number of reflections | 39246 | |
| <I/σ(I)> | 6.1 | 0.7 |
| Completeness [%] | 99.6 | 96.1 |
| Redundancy | 8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 295 | PEG 8000, Cacodylate, Acetate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | Cacodylate | ||
| 3 | 1 | 1 | Acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | Cacodylate | ||
| 7 | 1 | 2 | Acetate | ||
| 8 | 1 | 2 | H2O |
