2AXO
X-Ray Crystal Structure of Protein AGR_C_4864 from Agrobacterium tumefaciens. Northeast Structural Genomics Consortium Target AtR35.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.017, 57.296, 143.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.084 | 0.452 |
| Number of reflections | 37295 | |
| <I/σ(I)> | 23.4 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20 mM Tris (pH 7.5), 18% PEG 2kMME, 200 mM sodium formate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 293K |
