2AXC

Crystal structure of ColE7 translocation domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	120
Detector technology	CCD
Collection date	2004-06-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795, 0.9797, 0.9805
Spacegroup name	P 31 2 1
Unit cell lengths	59.038, 59.038, 132.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.520 - 1.700
R-factor	0.181
Rwork	0.181
R-free	0.20800
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	29942
<I/σ(I)>	68.5	12.2
Completeness [%]	99.1	100
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M Tris-HCl pH 8.5, 1.5M (NH4)2SO4, 12% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K