2AXB

Crystal Structure Analysis Of A 2-O-[2-(methoxy)ethyl]-2-thiothymidine Modified Oligodeoxynucleotide Duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	103
Collection date	2003-03-07
Wavelength(s)	.9873
Spacegroup name	P 21 21 21
Unit cell lengths	25.251, 45.002, 44.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.900 - 1.610
R-factor	0.19589
Rwork	0.196
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.534
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	12.900
High resolution limit [Å]	1.610
Number of reflections	6642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	Mini Screen, VAPOR DIFFUSION, HANGING DROP, temperature 287K