2AWU
Synapse associated protein 97 PDZ2 domain variant C378G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.519 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.220, 52.140, 52.640 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 19.650 - 2.440 |
| R-factor | 0.186 |
| Rwork | 0.180 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.560 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.029 | 0.094 |
| Number of reflections | 6219 | 1766 |
| <I/σ(I)> | 37.19 | 17.84 |
| Completeness [%] | 96.6 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.2M Sodium acetate, 0.1M Tris-HCl, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
