2AWO

Crystal structure of the ADP-Mg-bound E. Coli MALK (Crystallized with ADP-Mg)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Spacegroup name	P 1 21 1
Unit cell lengths	70.213, 101.975, 131.503
Unit cell angles	90.00, 90.73, 90.00

Refinement procedure

Resolution	29.930 - 2.800
R-factor	0.239
Rwork	0.239
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q1b
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	42086

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	5mM ADP-Mg2+, 0.2M Magnesium Formate, 15%~20% PEG3350, 15% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K