2AWC
deoxy-DcrH-Hr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.980, 33.120, 44.700 |
| Unit cell angles | 90.00, 96.02, 90.00 |
Refinement procedure
| Resolution | 18.860 - 2.200 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.250 |
| Number of reflections | 6560 | |
| <I/σ(I)> | 19.1 | 5.9 |
| Completeness [%] | 98.1 | 88.6 |
| Redundancy | 6.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | K2Pt(CN)4 , Tris-HCl, PEG 3350, CaCl2, isopropanol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
