2AWA

Crystal structure of DNA polymerase III, beta chain (EC 2.7.7.7) (np_344555.1) from STREPTOCOCCUS PNEUMONIAE TIGR4 at 2.50 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-02
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97934, 0.91837, 0.97923
Spacegroup name	P 1 21 1
Unit cell lengths	94.060, 70.720, 135.440
Unit cell angles	90.00, 91.04, 90.00

Refinement procedure

Resolution	30.000 - 2.500
Rwork	0.192
R-free	0.25000
Structure solution method	MAD, MOLECULAR REPLACEMENT
Starting model (for MR)	1mmi
RMSD bond length	0.012
RMSD bond angle	1.294
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.900	48.900	2.600
High resolution limit [Å]	2.500	5.750	2.500
Rmerge	0.088	0.678	0.678
Number of reflections	59570	9262	10004
<I/σ(I)>	8	16.04	1.85
Completeness [%]	91.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	7.5	277	10.0% iso-Propanol, 20.0% PEG-4000, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K