2AW6
Structure of a bacterial peptide pheromone/receptor complex and its mechanism of gene regulation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Collection date | 2005-03-02 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.546, 82.546, 380.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.21435 |
| Rwork | 0.212 |
| R-free | 0.25987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2awi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.420 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.126 | 0.415 |
| Number of reflections | 16419 | |
| <I/σ(I)> | 10 | 2.1 |
| Completeness [%] | 94.9 | 94.9 |
| Redundancy | 5.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | PEG4000, citrate-phosphate, pH 7.0, EVAPORATION, temperature 298K |
