2AW5
Crystal structure of a human malic enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.505, 136.963, 117.840 |
| Unit cell angles | 90.00, 121.75, 90.00 |
Refinement procedure
| Resolution | 37.300 - 2.500 |
| R-factor | 0.20984 |
| Rwork | 0.207 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.430 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.450 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 69400 | |
| Completeness [%] | 97.8 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | PEG 1K, Glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
