2AW5
Crystal structure of a human malic enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.505, 136.963, 117.840 |
Unit cell angles | 90.00, 121.75, 90.00 |
Refinement procedure
Resolution | 37.300 - 2.500 |
R-factor | 0.20984 |
Rwork | 0.207 |
R-free | 0.25590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.430 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.450 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 69400 | |
Completeness [%] | 97.8 | 97.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | PEG 1K, Glycerol, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |