2AVW
Crystal structure of monoclinic form of streptococcus Mac-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.986, 65.464, 137.244 |
| Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 104599 | |
| Completeness [%] | 84.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | 1.5M ammonium sulfate and 0.1M Ssodium citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
