2AVK
met-azido-DcrH-Hr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.450, 49.580, 69.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.530 |
| Rwork | 0.209 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.090 | 49.120 | 1.590 |
| High resolution limit [Å] | 1.530 | 6.930 | 1.550 |
| Rmerge | 0.083 | 0.044 | 0.202 |
| Number of reflections | 20792 | ||
| <I/σ(I)> | 3.9 | 13.3 | 3.6 |
| Completeness [%] | 99.9 | ||
| Redundancy | 13 | 10.7 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | ammonium sulfate, Tris-HCl buffer, sodium azide, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
