2AV5
Crystal structure of Pyrococcus furiosus Pop5, an archaeal Ribonuclease P protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.500, 107.500, 179.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.900 - 3.150 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26300 |
| Structure solution method | SIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 3.350 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 17989 | |
| Completeness [%] | 96.0 | 70 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | Imidazole, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
