2AUN
Active site His285Ala mutant of LD-carboxypeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.430, 78.012, 71.257 |
| Unit cell angles | 90.00, 104.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.213 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zrs |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.137 | 0.363 |
| Number of reflections | 21640 | |
| <I/σ(I)> | 7.8 | 3.2 |
| Completeness [%] | 99.3 | 99.2 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | citric acid, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
