2AUM
Active site Ser115Ala mutant of LD-carboxypeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.455, 77.843, 71.154 |
| Unit cell angles | 90.00, 104.53, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.24224 |
| Rwork | 0.242 |
| R-free | 0.24723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zrs |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.103 | 0.226 |
| Number of reflections | 21396 | |
| <I/σ(I)> | 8.2 | 3.6 |
| Completeness [%] | 98.6 | 96.4 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | citric acid , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
