2AUK
Structure of E. coli RNA polymerase beta' G/G' insert
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97156, 0.9794, 0.96384 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.030, 95.130, 86.070 |
| Unit cell angles | 90.00, 92.06, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.190 |
| Number of reflections | 43459 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | CHES/sodium formate/dioxane, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
