2AUD
Unliganded HincII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.740, 59.740, 164.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kc6 HincII bound to DNA monomer |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.141 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.033 | 0.297 |
| Number of reflections | 18087 | |
| <I/σ(I)> | 36.6 | 5 |
| Completeness [%] | 98.6 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | PEG 4K, Tris, NaCl, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
