2AUC
Structure of the Plasmodium MTIP-MyoA complex, a key component of the parasite invasion motor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97953 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.239, 95.239, 87.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.22938 |
| Rwork | 0.227 |
| R-free | 0.28326 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.939 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.739 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 13219 | |
| <I/σ(I)> | 21.1 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 298 | 20 % w/v PEG200, 0.05 M sodium acetate pH5.3, 1 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
