2AU5
Structure of a conserved domain from locus EF2947 from Enterococcus faecalis V583
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-08-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97956, 0.97973 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.779, 63.492, 43.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.800 - 2.100 |
| R-factor | 0.20261 |
| Rwork | 0.198 |
| R-free | 0.28877 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.426 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 7557 | |
| Completeness [%] | 97.6 | 99.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | PEG 1K, imidazole, calcium acetate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
