2ATZ
Crystal structure of protein HP0184 from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 77.093, 77.093, 118.187 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.20069 |
| Rwork | 0.198 |
| R-free | 0.25567 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.906 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.107 | 0.354 |
| Number of reflections | 14653 | |
| <I/σ(I)> | 63.1 | 4.95 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 19.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 297 | Tris, CL, PEG3350, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
