2ATW
Structure of a Mycobacterium tuberculosis NusA-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.564, 89.543, 100.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.250 |
| R-factor | 0.20934 |
| Rwork | 0.206 |
| R-free | 0.27267 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.734 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.058 | 0.410 |
| Number of reflections | 28167 | |
| <I/σ(I)> | 17.1 | 2.8 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | PEG 8000, Potassium dihydrogenphosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
