2ATP
Crystal structure of a CD8ab heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.668, 92.655, 79.330 |
| Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.400 |
| R-factor | 0.246 |
| Rwork | 0.242 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bqh |
| RMSD bond angle | 0.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 21001 | |
| <I/σ(I)> | 17.8 | 2.1 |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG4000, 0.2M (NH4)2SO4, 0.1 M Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
