2ATA
Structural Basis of DNA Recognition by p53 Tetramers (complex II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.649, 57.998, 77.983 |
| Unit cell angles | 83.35, 87.55, 73.50 |
Refinement procedure
| Resolution | 43.000 - 2.200 |
| R-factor | 0.149 |
| Rwork | 0.145 |
| R-free | 0.21500 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 2ca0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.030 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.255 |
| Number of reflections | 44444 | |
| <I/σ(I)> | 19.19 | 5.7 |
| Completeness [%] | 96.2 | 93.5 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | Lithium Chloride, PEG 3350, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Lithium Chloride | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | water | ||
| 4 | 1 | 2 | Lithium Chloride | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | water |
