2AT2

MOLECULAR STRUCTURE OF BACILLUS SUBTILIS ASPARTATE TRANSCARBAMOYLASE AT 3.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	258.500, 153.200, 51.900
Unit cell angles	90.00, 97.70, 90.00

Refinement procedure

Resolution	7.000 * - 3.000
R-factor	0.19
Rwork	0.190
RMSD bond length	0.028
RMSD bond angle	4.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.090 *
Total number of observations	154232 *
Number of reflections	30228 *
Completeness [%]	83.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution
2	1	drop		1.7 (M)
3	1	drop	Tris-HCl	0.1 (M)
4	1	drop	PEG200	2 (%)
5	1	reservoir		1.7 (M)
6	1	reservoir	Tris-HCl	0.1 (M)