2ASP
Structure of Rabbit Actin In Complex With Reidispongiolide C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-03 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.402, 54.689, 40.872 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.640 |
| R-factor | 0.16448 |
| Rwork | 0.163 |
| R-free | 0.19273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2asm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.039 | 0.226 |
| Number of reflections | 45723 | |
| <I/σ(I)> | 32.3 | 7.3 |
| Completeness [%] | 99.4 | 96.3 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50mM MES, pH 6.5, 12% methyl ether poly(ethylene glycol) 5000, 40mM MgCl2, 10% ethylene glycol, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
