2ASO
Structure of Rabbit Actin In Complex With Sphinxolide B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-28 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9641 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.182, 54.989, 103.520 |
| Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.700 |
| R-factor | 0.17511 |
| Rwork | 0.173 |
| R-free | 0.21347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.532 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.079 | 0.299 |
| Number of reflections | 36176 | |
| <I/σ(I)> | 29.1 | 3.8 |
| Completeness [%] | 99.4 | 95.1 |
| Redundancy | 4.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Na/MES/Acetate, pH 5.5, 12% methyl ether poly(ethylene glycol) 5000, 100mM CaCl2, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
