Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.857, 51.209, 174.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.150 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | |
| High resolution limit [Å] | 2.150 * | 2.150 * |
| Rmerge | 0.058 * | 0.261 * |
| Total number of observations | 121713 * | |
| Number of reflections | 19858 | |
| Completeness [%] | 93.8 | 83.8 * |
| Redundancy | 6.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 3.9 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | citrate | 10 (mM) | |
| 3 | 1 | drop | PEG8000 | 6 (%(w/v)) | |
| 4 | 1 | reservoir | PEG8000 | 12 (%) | |
| 5 | 1 | reservoir | citrate | 10 (mM) |
