2ASB
Structure of a Mycobacterium tuberculosis NusA-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 71.416, 71.416, 115.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.22246 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0r |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.608 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.042 | 0.540 |
| Number of reflections | 47092 | |
| <I/σ(I)> | 28.4 | 2.1 |
| Completeness [%] | 97.1 | 86.3 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | PEG 4000, Lithium sulphate, Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
