2ARU
Crystal structure of lipoate-protein ligase A bound with ATP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Detector technology | CCD |
| Collection date | 2004-12-08 |
| Detector | BRUKER PROTEUM 300 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.532, 62.809, 46.448 |
| Unit cell angles | 90.00, 111.34, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 10079 | |
| Completeness [%] | 98.8 | 93.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 100mM sodium citrate at pH 5.5, 20%(v/v) iso-propanol, 20%(w/v) PEG 3350, 100mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
