2ARC
ESCHERICHIA COLI REGULATORY PROTEIN ARAC COMPLEXED WITH L-ARABINOSE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.750, 93.840, 50.330 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.500 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.23200 |
| Structure solution method | SINGLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | |
| Number of reflections | 58716 | |
| <I/σ(I)> | 10.9 | 2.5 |
| Completeness [%] | 94.7 | 90.2 |
| Redundancy | 5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.25 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | magnesium acetate | 40 (mM) |
