2ARA
APO FORM OF ESCHERICHIA COLI REGULATORY PROTEIN ARAC
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | FUJI |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 57.370, 57.370, 83.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.33700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2arc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 25.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.820 |
| High resolution limit [Å] | 2.700 | 1.800 |
| Rmerge | 0.065 | 0.065 |
| Total number of observations | 51550 * | |
| Number of reflections | 4676 | |
| <I/σ(I)> | 16.5 | 3 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 11 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | PROTEIN WAS CRYSTALLIZED BY HANGING-DROP VAPOR DIFFUSION FROM 20% PEG 4000, 0.1M TRIS-HCL, PH 9.0, 5 MM KCL, 0.2 M SODIUM ACETATE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 0.2 (M) |
