2AR7
Crystal structure of human adenylate kinase 4, AK4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.968 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.738, 95.514, 140.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.150 |
| R-factor | 0.16934 |
| Rwork | 0.166 |
| R-free | 0.22995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bbw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.455 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.950 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 32401 | |
| <I/σ(I)> | 19.5 | 2.1 |
| Completeness [%] | 97.2 | 82.1 |
| Redundancy | 6.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | sodium succinate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
