2AR0

Crystal structure of Type I restriction enzyme EcoKI M protein (EC 2.1.1.72) (M.EcoKI)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-17
Detector	MARRESEARCH
Wavelength(s)	0.975
Spacegroup name	P 41
Unit cell lengths	105.427, 105.427, 138.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.800
R-factor	0.224
Rwork	0.224
R-free	0.26100
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	70488
<I/σ(I)>	8.6
Completeness [%]	96.6	95.7
Redundancy	2.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	291	2.15M (NH2)2SO4, 0.1M Na Citrate, pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 291K