2AQP
CU/ZN superoxide dismutase from neisseria meningitidis E73A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 96 |
| Collection date | 2001-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.874, 48.697, 49.042 |
| Unit cell angles | 90.00, 109.57, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.300 |
| R-factor | 0.1353 |
| Rwork | 0.135 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD-TYPE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.045 | 0.250 |
| Number of reflections | 75512 | |
| <I/σ(I)> | 14.6 | 4.54 |
| Completeness [%] | 94.7 | 82.7 |
| Redundancy | 4.03 | 3.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
