2AQ5
Crystal Structure of Murine Coronin-1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.9797, 0.9570 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.996, 80.443, 88.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.16 |
| Rwork | 0.158 |
| R-free | 0.19200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 38545 | |
| <I/σ(I)> | 33.9 | 11.8 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 12.1 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 292 | 30% PEG-MME 2000, 150 mM potassium chloride, 5 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 292K, pH 8.0 |
