2APL
Crystal structure of protein PG0816 from Porphyromonas gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.947, 55.159, 80.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.600 - 2.010 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.23367 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.157 |
| Number of reflections | 12416 | |
| <I/σ(I)> | 52.4 | 11.2 |
| Completeness [%] | 95.8 | 71.9 |
| Redundancy | 6.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Ammonium Acetate, Bis-Tris, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
