2APH
Crystal structure of human PGRP-IalphaC in complex with muramyl pentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source details | OTHER |
| Temperature [K] | 165 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.860, 58.630, 70.400 |
| Unit cell angles | 90.00, 93.65, 90.00 |
Refinement procedure
| Resolution | 37.780 - 2.100 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1SK3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | |
| Number of reflections | 20828 | |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 99.6 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG2000 MME, Nickel sulfate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
