2AP3
1.6 A Crystal Structure of a Conserved Protein of Unknown Function from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9798,0.94656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.240, 45.762, 72.485 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.226 |
| Rwork | 0.224 |
| R-free | 0.27188 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.147 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 | 0.114 |
| Number of reflections | 27203 | |
| <I/σ(I)> | 36.68 | 6.7 |
| Completeness [%] | 97.3 | 92.4 |
| Redundancy | 9.5 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M Bis/Tris propane, 2.0M NaAc, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
