2AOS
Protein-protein Interactions of protective signalling factor: Crystal structure of ternary complex involving signalling protein from goat (SPG-40), tetrasaccharide and a tripeptide Trp-pro-Trp at 2.9 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2005-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.871, 66.512, 107.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.000 - 2.900 |
R-factor | 0.19462 |
Rwork | 0.192 |
R-free | 0.23866 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ljy |
RMSD bond length | 0.015 |
RMSD bond angle | 2.090 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 3.120 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 10278 | |
<I/σ(I)> | 7.1 | 2 |
Completeness [%] | 96.8 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 25 mM TRIS-HCL, 50 mM NACL, 19% ETHANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |