2AO9
Structural Genomics, The crystal structure of a Phage protein (phBC6A51) from Bacillus cereus ATCC 14579
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-28 |
Detector | SBC-3 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.016, 150.481, 99.975 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.100 - 1.900 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.23581 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.072 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.090 | 0.610 |
Number of reflections | 134863 | |
<I/σ(I)> | 22.76 | 2 |
Completeness [%] | 99.6 | 99.1 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 1.26M (NH)4SO4, 0.1M CHES. 0.2M NaCl, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |