2ANE
Crystal structure of N-terminal domain of E.Coli Lon Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-08-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.610, 53.445, 111.973 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.030 |
| Rwork | 0.212 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.860 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.352 | |
| Number of reflections | 63721 | |
| <I/σ(I)> | 38 | 3.1 |
| Completeness [%] | 95.8 | 78.3 |
| Redundancy | 4.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG8000, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
