2ANC
Crystal Structure Of Unliganded Form Of Oligomeric E.coli Guanylate Kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.238, 108.238, 273.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.900 - 3.200 |
| R-factor | 0.25722 |
| Rwork | 0.256 |
| R-free | 0.28298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2AN9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.071 | 0.225 |
| Number of reflections | 27681 | |
| <I/σ(I)> | 26.9 | 11.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 10.6 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 16% (w/v) PEG 10000, 0.08M sodium hepes, 0.1M sodium cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
