2ANB
Crystal Structure Of Oligomeric E.coli Guanylate Kinase In Complex With GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 106.728, 106.728, 155.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 2.900 |
| R-factor | 0.20509 |
| Rwork | 0.203 |
| R-free | 0.24181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2an9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.435 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.074 | 0.439 |
| Number of reflections | 7689 | |
| <I/σ(I)> | 23.6 | 5.8 |
| Completeness [%] | 99.0 | 99.1 |
| Redundancy | 10.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 1.4M ammonium sulfate, 0.2M lithium chloride, 0.1M sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
