2AN6
Protein-peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.117 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.300, 100.000, 103.400 |
| Unit cell angles | 90.00, 104.30, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.24064 |
| Rwork | 0.237 |
| R-free | 0.30498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.757 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.508 |
| Number of reflections | 23606 | |
| <I/σ(I)> | 13 | 1.3 |
| Completeness [%] | 99.2 | 95.8 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | magnesium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
