2AN1
Structural Genomics, The crystal structure of a putative kinase from Salmonella typhimurim LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-14 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.535, 99.323, 144.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.20163 |
| Rwork | 0.200 |
| R-free | 0.23262 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.226 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.523 |
| Number of reflections | 94632 | |
| <I/σ(I)> | 26 | 1.87 |
| Completeness [%] | 97.2 | 81 |
| Redundancy | 8.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 1.5M Ammonium Sulfate, 0.1 M Sodium Acetate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
